Myer has slashed 90 employees from its Melbourne head office roster and redeployed another 45.
The latest cull follows a series of staff cuts, including 35 executive roles in January and 80 jobs over 2018/2019 across senior management, marketing, merchandising and store administration.
In a statement, Myer said the decision is in response to the changing landscape.
"We have made a number of changes across the workforce, as we focus on delivering to the changing retail landscape.
"This review however does affects approx. 90 roles who will leave the business. The number of roles leaving the business is less than 1% of team members across our workforce and will include functions across management/store management, business support, administration and duplicate roles.
"To minimise the impacts to our team members we also successfully redeployed over 45 people to new or redefined roles.
"As part of these plans we will continue to invest across our online and supply chain functions."
The news comes as insurer QBE cuts cover for suppliers to Myer, citing the department store sector as a high risk category during COVID-19.
While Myer has not issued a formal trading update since the onset of COVID-19, experts are predicting sales falls of as much as 50%.
In its last trading announcement Myer confirmed cost reductions would continue to mitigate the current trading environment.
“Myer is continuing to take all necessary measure to minimise costs, including engaging in ongoing discussions with suppliers and landlords.”
Myer's first half performance for fiscal 2020 revealed net profit after tax fell 26.9% to $28.1 million in the half, including restructuring and redundancy costs.