Myer will end its experiment in specialty retailing as part of a series of store closures.
Myer will be exiting its Top Ryde City (NSW) shopping centre store next month.
The department store will also close three stand-alone specialty stores in Melbourne's North.
The stores, trialled over a 12 month period, stocked Myer exclusive brands across womenswear, menswear and childrenswear.
Myer CEO Richard Umbers confirmed the stores will close in August to focus on bricks-and-mortar and online.
The opportunity to exit Top Ryde has been facilitated by plans to develop the centre.
A review of Myer One data indicated a majority of customers choose to shop at nearby Myer stores.
"We have recently undertaken an extensive analysis of our current and future customer base on a catchment by catchment basis that now informs our decision making," Umbers said.